Forming next-generation antibody-nanoparticle conjugates through the oriented installation of non-engineered antibody fragments.

ABSTRACT: The successful development of targeted nanotherapeutics is contingent upon the conjugation of therapeutic nanoparticles to target-specific ligands, with particular emphasis being placed on antibody-based ligands. Thus, new methods that enable the covalent and precise installation of targeting antibodies to nanoparticle surfaces are greatly desired, especially those which do not rely on costly and time-consuming antibody engineering techniques. Herein we present a novel method for the highly controlled and oriented covalent conjugation of non-engineered antibody F(ab) fragments to PLGA-PEG nanoparticles using disulfide-selective pyridazinedione linkers and strain-promoted alkyne-azide click chemistry. Exemplification of this method with trastuzumab and cetuximab showed significant improveme

SUBMITTER: Greene MK 

PROVIDER: S-EPMC5869316 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature