Fresh is best: Accurate SNP genotyping from koala scats.

ABSTRACT: Maintaining genetic diversity is a crucial component in conserving threatened species. For the iconic Australian koala, there is little genetic information on wild populations that is not either skewed by biased sampling methods (e.g., sampling effort skewed toward urban areas) or of limited usefulness due to low numbers of microsatellites used. The ability to genotype DNA extracted from koala scats using next-generation sequencing technology will not only help resolve location sample bias but also improve the accuracy and scope of genetic analyses (e.g., neutral vs. adaptive genetic diversity, inbreeding, and effective population size). Here, we present the successful SNP genotyping (1272 SNP loci) of koala DNA extracted from scat, using a proprietary DArTseq^™ protocol. We comp

SUBMITTER: Schultz AJ 

PROVIDER: S-EPMC5869377 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature