Survival of Anaerobic Fe²⁺ Stress Requires the ClpXP Protease.

ABSTRACT: Shewanella oneidensis strain MR-1 is a versatile bacterium capable of respiring extracellular, insoluble ferric oxide minerals under anaerobic conditions. The respiration of iron minerals results in the production of soluble ferrous ions, which at high concentrations are toxic to living organisms. It is not fully understood how Fe²⁺ is toxic to cells anaerobically, nor is it fully understood how S. oneidensis is able to resist high levels of Fe²⁺ Here we describe the results of a transposon mutant screen and subsequent deletion of the genes clpX and clpP in S. oneidensis, which demonstrate that the protease ClpXP is required for anaerobic Fe²⁺ resistance. Many cellular processes are known to be regulated by ClpXP, includin

SUBMITTER: Bennett BD 

PROVIDER: S-EPMC5869471 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature