Deciphering the Human Virome with Single-Virus Genomics and Metagenomics.

ABSTRACT: Single-cell genomics has unveiled the metabolic potential of dominant microbes inhabiting different environments, including the human body. The lack of genomic information for predominant microbes of the human body, such as bacteriophages, hinders our ability to answer fundamental questions about our viral communities. Here, we applied single-virus genomics (SVGs) to natural human salivary samples in combination with viral metagenomics to gain some insights into the viral community structure of the oral cavity. Saliva samples were processed for viral metagenomics (n = 15) and SVGs (n = 3). A total of 1328 uncultured single viruses were sorted by fluorescence-activated virus sorting followed by whole genome amplification. Sequencing of 24 viral single amplified genomes (vSAGs) showed that h

SUBMITTER: de la Cruz Pena MJ 

PROVIDER: S-EPMC5869506 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature