Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation.

ABSTRACT: Transport of cellular cargo by molecular motors requires directionality to ensure proper biological functioning. During sporulation in Bacillus subtilis, directionality of chromosome transport is mediated by the interaction between the membrane-bound DNA translocase SpoIIIE and specific octameric sequences (SRS). Whether SRS regulate directionality by recruiting and orienting SpoIIIE or by simply catalyzing its translocation activity is still unclear. By using atomic force microscopy and single-round fast kinetics translocation assays we determined the localization and dynamics of diffusing and translocating SpoIIIE complexes on DNA with or without SRS. Our findings combined with mathematical modelling revealed that SpoIIIE directionality is not regulated by protein recruitment to SRS but

SUBMITTER: Chara O 

PROVIDER: S-EPMC5869595 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature