Respiromics - An integrative analysis linking mitochondrial bioenergetics to molecular signatures.

ABSTRACT:

Objective

Energy metabolism is challenged upon nutrient stress, eventually leading to a variety of metabolic diseases that represent a major global health burden.

Methods

Here, we combine quantitative mitochondrial respirometry (Seahorse technology) and proteomics (LC-MS/MS-based total protein approach) to understand how molecular changes translate to changes in mitochondrial energy transduction during diet-induced obesity (DIO) in the liver.

Results

The integrative analysis reveals that significantly increased palmitoyl-carnitine respiration is supported by an array of proteins enriching lipid metabolism pathways. Upstream of the respiratory chain, the increased capacity for ATP synthesis during DIO associates strongest to mitochondrial uptake of pyruvate, which is r

SUBMITTER: Walheim E 

PROVIDER: S-EPMC5869730 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature