Hypothalamic miR-219 regulates individual metabolic differences in response to diet-induced weight cycling.

ABSTRACT: Consumption of a low calorie diet is the most common approach to lose weight. While generally effective at first, it is frequently followed by a relapse where the pre-diet weight is regained, and often exceeded. This pattern of repeated weight loss/regain is referred to as weight cycling and the resulting metabolic response varies greatly between individuals.

Objective

We attempted to address the issue of individual differences in the response to weight cycling in male mice.

Methods

We first exposed adult wild type mice to repeated cycles of high/low fat food. Next, using a lentiviral approach, we knocked-down or over-expressed miR-219 in the ventromedial hypothalamus (VMH) of an additional mouse cohort and performed a full metabolic assessment.

Results

Exposure of wil

SUBMITTER: Schroeder M 

PROVIDER: S-EPMC5870106 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature