Scalable Production and Cryostorage of Organoids Using Core-Shell Decoupled Hydrogel Capsules.

ABSTRACT: Organoids, organ-mimicking multicellular structures derived from pluripotent stem cells or organ progenitors, have recently emerged as an important system for both studies of stem cell biology and development of potential therapeutics; however, a large-scale culture of organoids and cryopreservation for whole organoids, a prerequisite for their industrial and clinical applications, has remained a challenge. Current organoid culture systems relying on embedding the stem or progenitor cells in bulk extracellular matrix (ECM) hydrogels (e.g., Matrigel™) have limited surface area for mass transfer and are not suitable for large-scale productions. Here, we demonstrate a capsule-based, scalable organoid production and cryopreservation platform. The capsules have a core-shell structure where the

SUBMITTER: Lu YC 

PROVIDER: S-EPMC5870136 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature