Effects of the S42 residue of the H1N1 swine influenza virus NS1 protein on interferon responses and virus replication.

ABSTRACT:

Background

The influenza A virus non-structural protein 1 (NS1) is a multifunctional protein that plays an important role in virus replication, virulence and inhibition of the host antiviral immune response. In the avian influenza virus or human influenza virus, specific amino acids of NS1 have been shown to be important for the virus to antagonize host antiviral defenses and promote viral replication. However, little research has been reported regarding the swine influenza virus (SIV) NS1 protein.

Methods

To study the effects of the key amino acids of NS1, we rescued NS1 mutants (S42P, D92E, and S42P/D92E) of the A/swine/Shanghai/3/2014(H1N1) strain and compared their replication ability and cytokine production as well as the intracellular localization in cultured cells.

SUBMITTER: Cheng J 

PROVIDER: S-EPMC5870223 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature