Analysis of SNPs of MC4R, GNB3 and FTO gene polymorphism in obese Saudi subjects.

ABSTRACT:

Background

The goal of this study was to analyze the association between the FTO rs17817449 (G>T), G protein beta3 subunit (GNB3) C825T and Melanocortin 4 receptor (MC4R) A822G single nucleotide polymorphism (SNP) with obesity in Saudi subjects.

Methods

The subjects were divided into 2 groups according to BMI: Obese (BMI> 29.9) and non- obese control (BMI<24.9). Genotyping of the target genes were determined by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP).

Results

We demonstrated the association of the FTO genotype TT with increased weight, BMI and leptin levels in both males and females. However, there was no association of genotype TT with fasting blood glucose, triglycerides and cholesterol levels. Regarding G

SUBMITTER: Moselhy SS 

PROVIDER: S-EPMC5870267 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature