Experimental infection of pigs with H1 and H3 influenza A viruses of swine by using intranasal nebulization.

ABSTRACT:

Background

Experimental infection of pigs via direct intranasal or intratracheal inoculation has been mainly used to study the infectious process of influenza A viruses of swine (IAVs-S). Nebulization is known to be an alternative method for inoculating pigs with IAVs-S, because larger quantities of virus potentially can be delivered throughout the respiratory tract. However, there is very little data on the experimental infection of pigs by inhalation using nebulizer. In the current study, we used intranasal nebulization to inoculate pigs with 9 different IAVs-S-3 H1N1, 2 H1N2, and 4 H3N2 strains. We then assessed the process of infection by evaluating the clinical signs, nasal and oral viral shedding, and seroconversion rates of the pigs inoculated.

Results

Lethargy and sn

SUBMITTER: Takemae N 

PROVIDER: S-EPMC5870511 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature