Species identification approach for both raw materials and end products of herbal supplements from Tinospora species.

ABSTRACT:

Background

Nowadays herbal products used in traditional medicine are sold in processed forms and thus morphological authentication is almost impossible. With herbal industry rapidly growing size, consumer safety becomes an important issue that requires special attention. Identification of herbal species in the products is therefore needed.

Methods

Sequences from the selected regions (matK, rbcL, trnL and ITS1) were retrieved and analysed. Then the most suitable barcode was assessed for discrimination of T. crispa from closely related species by HRM analysis and used in authentication of commercial products.

Results

The ITS1 barcode was found to be the suitable primer as melting data from the HRM assay proved to be capable of distinguishing T. crispa from its related s

SUBMITTER: Osathanunkul M 

PROVIDER: S-EPMC5870811 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature