Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models.

ABSTRACT:

Background

Patient-derived tumor models are the new standard for pre-clinical drug testing and biomarker discovery. However, the emerging technology of primary pancreatic cancer organoids has not yet been broadly implemented in research, and complex organotypic models using organoids in co-culture with stromal and immune cellular components of the tumor have yet to be established. In this study, our objective was to develop and characterize pancreatic cancer organoids and multi-cell type organotypic co-culture models to demonstrate their applicability to the study of pancreatic cancer.

Methods

We employed organoid culture methods and flow cytometric, cytologic, immunofluorescent and immunohistochemical methods to develop and characterize patient-derived pancreatic cancer org

SUBMITTER: Tsai S 

PROVIDER: S-EPMC5870823 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature