Oligohistidine and targeting peptide functionalized TAT-NLS for enhancing cellular uptake and promoting angiogenesis in vivo.

ABSTRACT:

Background

Gene therapy has been developed and used in medical treatment for many years, especially for the enhancement of endothelialization and angiogenesis. But slow endosomal escape rate is still one of the major barriers to successful gene delivery. In order to evaluate whether introducing oligohistidine (H_n) sequence into gene carriers can promote endosomal escape and gene transfection or not, we designed and synthesized Arg-Glu-Asp-Val (REDV) peptide functionalized TAT-NLS-H_n (TAT: typical cell-penetrating peptide, NLS: nuclear localization signals, H_n: oligohistidine sequence, n: 4, 8 and 12) peptides with different H_n sequence lengths. pEGFP-ZNF580 (pZNF580) was condensed by these peptides to form gene complexes, which were used

SUBMITTER: Li Q 

PROVIDER: S-EPMC5870920 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature