Affinity-purified DNA-based mutation profiles of endometriosis-related ovarian neoplasms in Japanese patients.

ABSTRACT:

Aim

Endometriosis-related ovarian neoplasms (ERONs) have recently attracted considerable attention; however, the prevalence and patterns of ARID1A and POLE mutations in ERONs have not been studied in detail. The aim of this study was to investigate not only the carcinogenesis of ERONs, but also the prognostic significance of several gene mutations in this cohort. We used DNA purified from only tumor epithelial cells, from which fibroblasts were removed, using a specific method we called "liquid microdissection".

Methods

Tissue samples from 22 ovarian carcinomas (13 endometrioid, and nine clear cell) were used. Tumor cells were isolated using a cell sorting system and DNA was purified from tumor epithelial cells. Nucleotide sequencing was conducted to analyze th

SUBMITTER: Ishikawa M 

PROVIDER: S-EPMC5871076 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature