Molecular dissection of engraftment in a xenograft model of myelodysplastic syndromes.

ABSTRACT: Myelodysplastic syndromes (MDS) are oligoclonal disorders of the hematopoietic stem cells (HSC). Recurrent gene mutations are involved in the MDS physiopathology along with the medullar microenvironment. To better study the heterogeneity of MDS, it is necessary to create patient derived xenograft (PDX). We have reproduced a PDX model by xenografting HSC (CD34⁺) and mesenchymal stromal cells (MSC) in NOD/SCID/IL2rγ^-/- mice with primary samples from one RAEB2, two RAEB1 and one RARS patients harboring karyotype abnormalities and gene mutations. The average human chimerisms ranged from 59.7% to 0.0175% for the 4 patients. Secondary grafts (G2) were only performed for mice derived from the RAEB2 patient and the average human chimerism was 53.33%. G1 mice 1 and 2, and thei

SUBMITTER: Meunier M 

PROVIDER: S-EPMC5871091 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature