An optimised spectrophotometric assay for convenient and accurate quantitation of intracellular iron from iron oxide nanoparticles.

ABSTRACT: We report the development and optimisation of an assay for quantitating iron from iron oxide nanoparticles in biological matrices by using ferene-s, a chromogenic compound. The method is accurate, reliable and can be performed with basic equipment common to many laboratories making it convenient and inexpensive. The assay we have developed is suited for quantitation of iron in cell culture studies with iron oxide nanoparticles, which tend to manifest low levels of iron. The assay was validated with standard reference materials and with inductively coupled plasma-mass spectrometry (ICP-MS) to accurately measure iron concentrations ∼1 × 10^-6 g in about 1 × 10⁶ cells (∼1 × 10^-12 g Fe per cell). The assay requires preparation and use of a working solution to wh

SUBMITTER: Hedayati M 

PROVIDER: S-EPMC5871594 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature