Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase.

ABSTRACT: G protein-coupled receptors (GPCRs), G proteins and adenylyl cyclase (AC) comprise one of the most studied transmembrane cell signaling pathways. However, it is unknown whether the ligand-dependent interactions between these signaling molecules are based on random collisions or the rearrangement of pre-coupled elements in a macromolecular complex. Furthermore, it remains controversial whether a GPCR homodimer coupled to a single heterotrimeric G protein constitutes a common functional unit. Using a peptide-based approach, we here report evidence for the existence of functional pre-coupled complexes of heteromers of adenosine A_2A receptor and dopamine D₂ receptor homodimers coupled to their cognate Gs and Gi proteins and to subtype 5 AC. We also demonstrate that this m

SUBMITTER: Navarro G 

PROVIDER: S-EPMC5871782 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature