Use of a Regression Model to Study Host-Genomic Determinants of Phage Susceptibility in MRSA.

ABSTRACT: Staphylococcus aureus is a major agent of nosocomial infections. Especially in methicillin-resistant strains, conventional treatment options are limited and expensive, which has fueled a growing interest in phage therapy approaches. We have tested the susceptibility of 207 clinical S. aureus strains to 12 (nine monovalent) different therapeutic phage preparations and subsequently employed linear regression models to estimate the influence of individual host gene families on resistance to phages. Specifically, we used a two-step regression model setup with a preselection step based on gene family enrichment. We show that our models are robust and capture the data's underlying signal by comparing their performance to that of models build on randomized data. In doing so, we have identified 16

SUBMITTER: Zschach H 

PROVIDER: S-EPMC5872120 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature