Comparison of three PCR-based assays for SNP genotyping in plants.

ABSTRACT:

Background

PCR allelic discrimination technologies have broad applications in the detection of single nucleotide polymorphisms (SNPs) in genetics and genomics. The use of fluorescence-tagged probes is the leading method for targeted SNP detection, but assay costs and error rates could be improved to increase genotyping efficiency. A new assay, rhAmp, based on RNase H2-dependent PCR (rhPCR) combined with a universal reporter system attempts to reduce error rates from primer/primer and primer/probe dimers while lowering costs compared to existing technologies. Before rhAmp can be widely adopted, more experimentation is required to validate its effectiveness versus established methods.

Results

The aim of this study was to compare the accuracy, sensitivity and costs of TaqMan, K

SUBMITTER: Broccanello C 

PROVIDER: S-EPMC5872507 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature