Establishment of a universal and rational gene detection strategy through three-way junction-based remote transduction.

ABSTRACT: The polymerase chain reaction and many isothermal amplifications are able to achieve super gene amplification. Unfortunately, most commonly-used transduction methods, such as dye staining and Taqman-like probing, still suffer from shortcomings including false signals or difficult probe design, or are incompatible with multi-analysis. Here a universal and rational gene detection strategy has been established by translating isothermal amplicons to enzyme-free strand displacement circuits via three-way junction-based remote transduction. An assistant transduction probe was imported to form a partial hybrid with the target single-stranded nucleic acid. After systematic optimization the hybrid could serve as an associative trigger to activate a downstream circuit detector via a strand displacem

SUBMITTER: Tang Y 

PROVIDER: S-EPMC5872807 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature