Deep Sequencing of Urinary RNAs for Bladder Cancer Molecular Diagnostics.

ABSTRACT: Purpose: The majority of bladder cancer patients present with localized disease and are managed by transurethral resection. However, the high rate of recurrence necessitates lifetime cystoscopic surveillance. Developing a sensitive and specific urine-based test would significantly improve bladder cancer screening, detection, and surveillance.Experimental Design: RNA-seq was used for biomarker discovery to directly assess the gene expression profile of exfoliated urothelial cells in urine derived from bladder cancer patients (n = 13) and controls (n = 10). Eight bladder cancer specific and 3 reference genes identified by RNA-seq were quantitated by qPCR in a training cohort of 102 urine samples. A diagnostic model based on the training cohort was constructed usin

SUBMITTER: Sin MLY 

PROVIDER: S-EPMC5873297 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature