A combinatorial approach to synthetic transcription factor-promoter combinations for yeast strain engineering.

ABSTRACT: Despite the need for inducible promoters in strain development efforts, the majority of engineering in Saccharomyces cerevisiae continues to rely on a few constitutively active or inducible promoters. Building on advances that use the modular nature of both transcription factors and promoter regions, we have built a library of hybrid promoters that are regulated by a synthetic transcription factor. The hybrid promoters consist of native S. cerevisiae promoters, in which the operator regions have been replaced with sequences that are recognized by the bacterial LexA DNA binding protein. Correspondingly, the synthetic transcription factor (TF) consists of the DNA binding domain of the LexA protein, fused with the human estrogen binding domain and the viral activator domain, VP16. The resulti

SUBMITTER: Dossani ZY 

PROVIDER: S-EPMC5873372 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature