The role of intestinal microbiota in murine models of acetaminophen-induced hepatotoxicity.

ABSTRACT:

Background & aims

Variations in intestinal microbiota may influence acetaminophen metabolism. This study aimed to determine whether intestinal microbiota are a source of differential susceptibility to acetaminophen-induced hepatotoxicity.

Methods

Conventionally housed C3H/HeH (CH) and C3H/HeH germ-free (GF) mice were administered a 200 mg/kg IP dose of acetaminophen. The severity of hepatotoxicity at 8 h was assessed by histology and biochemical indices. A urinary metabolic profile was obtained using (1) H-NMR. Baseline hepatic glutathione content and CYP2E1 expression were quantified. An additional group of C3H/HeJ (LPS-r) mice were assessed to determine the contribution of LPS/TLR4 signalling.

Results

Baseline glutathione levels were significantly reduced (P = 0.03)

SUBMITTER: Possamai LA 

PROVIDER: S-EPMC5873516 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature