Integrated RNA and DNA sequencing reveals early drivers of metastatic breast cancer.

ABSTRACT: Breast cancer metastasis remains a clinical challenge, even within a single patient across multiple sites of the disease. Genome-wide comparisons of both the DNA and gene expression of primary tumors and metastases in multiple patients could help elucidate the underlying mechanisms that cause breast cancer metastasis. To address this issue, we performed DNA exome and RNA sequencing of matched primary tumors and multiple metastases from 16 patients, totaling 83 distinct specimens. We identified tumor-specific drivers by integrating known protein-protein network information with RNA expression and somatic DNA alterations and found that genetic drivers were predominantly established in the primary tumor and maintained through metastatic spreading. In addition, our analyses revealed that most

SUBMITTER: Siegel MB 

PROVIDER: S-EPMC5873890 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature