The Molecular and Genetic Characterization of Second Chromosome Balancers in Drosophila melanogaster.

ABSTRACT: Balancer chromosomes are multiply inverted and rearranged chromosomes used in Drosophila melanogaster for many tasks, such as maintaining mutant alleles in stock and complex stock construction. Balancers were created before molecular characterization of their breakpoints was possible, so the precise locations of many of these breakpoints are unknown. Here, we report or confirm the positions of the 14 euchromatic breakpoints on the 2^nd chromosome balancers SM1, SM5, CyO, and SM6a This total includes three breakpoints involved in a complex rearrangement on SM5 that is associated with the duplication of two genomic regions. Unbiased sequencing of several balancers allowed us to identify stocks with incorrectly identified balancers as

SUBMITTER: Miller DE 

PROVIDER: S-EPMC5873907 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature