Targeted Long-Read Sequencing of a Locus Under Long-Term Balancing Selection in Capsella.

ABSTRACT: Rapid advances in short-read DNA sequencing technologies have revolutionized population genomic studies, but there are genomic regions where this technology reaches its limits. Limitations mostly arise due to the difficulties in assembly or alignment to genomic regions of high sequence divergence and high repeat content, which are typical characteristics for loci under strong long-term balancing selection. Studying genetic diversity at such loci therefore remains challenging. Here, we investigate the feasibility and error rates associated with targeted long-read sequencing of a locus under balancing selection. For this purpose, we generated bacterial artificial chromosomes (BACs) containing the Brassicaceae S-locus, a region under strong negative frequency-dependent selection which

SUBMITTER: Bachmann JA 

PROVIDER: S-EPMC5873921 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature