Active BRAF-V600E is the key player in generation of a sessile serrated polyp-specific DNA methylation profile.

ABSTRACT:

Background

Sessile serrated polyps (SSPs) have emerged as important precursors for a large number of sporadic colorectal cancers. They are difficult to detect during colonoscopy due to their flat shape and the excessive amounts of secreted mucin that cover the polyps. The underlying genetic and epigenetic basis for the emergence of SSPs is largely unknown with existing genetic studies confined to a limited number of oncogenes and tumor suppressors. A full characterization of the genetic and epigenetic landscape of SSPs would provide insight into their origin and potentially offer new biomarkers useful for detection of SSPs in stool samples.

Methods

We used a combination of genome-wide mutation detection, exome sequencing and DNA methylation profiling (via methyl-array and wh

SUBMITTER: Dehghanizadeh S 

PROVIDER: S-EPMC5873940 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature