Investigating Potential Chromosomal Rearrangements during Laboratory Culture of Neisseria gonorrhoeae.

ABSTRACT: Comparisons of genome sequence data between different strains and isolates of Neisseria spp., such as Neisseria gonorrhoeae, reveal that over the evolutionary history of these organisms, large scale chromosomal rearrangements have occurred. Factors within the genomes, such as repetitive sequences and prophage, are believed to have contributed to these observations. However, the timescale in which rearrangements occur is not clear, nor whether it might be expected for them to happen in the laboratory. In this study, N. gonorrhoeae was repeatedly passaged in the laboratory and assessed for large scale chromosomal rearrangements. Using gonococcal strain NCCP11945, for which there is a complete genome sequence, cultures were passaged for eight weeks in the laboratory. The

SUBMITTER: Spencer-Smith R 

PROVIDER: S-EPMC5874624 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature