Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans.

ABSTRACT:

Background

Advances in automated image-based microscopy platforms coupled with high-throughput liquid workflows have facilitated the design of large-scale screens utilising multicellular model organisms such as Caenorhabditis elegans to identify genetic interactions, therapeutic drugs or disease modifiers. However, the analysis of essential genes has lagged behind because lethal or sterile mutations pose a bottleneck for high-throughput approaches, and a systematic way to analyse genetic interactions of essential genes in multicellular organisms has been lacking.

Results

In C. elegans, non-conditional lethal mutations can be maintained in heterozygosity using chromosome balancers, commonly expressing green fluorescent protein (GFP) in the pharynx. However, gene expression or

SUBMITTER: Hernando-Rodriguez B 

PROVIDER: S-EPMC5875015 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature