Hydrogen peroxide-triggered gene silencing in mammalian cells through boronated antisense oligonucleotides.

ABSTRACT: Hydrogen peroxide (H₂O₂) is a reactive oxygen species (ROS) involved in various diseases, including neurodegeneration, diabetes, and cancer. Here, we introduce a new approach to use H₂O₂ to modulate specific gene expression in mammalian cells. H₂O₂-responsive nucleoside analogues, in which the Watson-Crick faces of the nucleobases are caged by arylboronate moieties, were synthesized. One of these analogues, boronated thymidine (dT^B ), was incorporated into oligodeoxynucleotides (ODNs) using an automated DNA synthesizer. The hybridization ability of this boronated ODN to complementary RNA was clearly switched in the off-to-on direction upon H₂O₂ addition. Furthermore, we demonstrated H<

SUBMITTER: Mori S 

PROVIDER: S-EPMC5875086 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature