Ischemic Stroke Alters the Expression of the Transcribed Ultraconserved Regions of the Genome in Rat Brain.

ABSTRACT:

Background and purpose

Human and rodent genomes diverged ≈75 million years ago. However, 481 regions of their genomes (200-779 nucleotide each) remained absolutely conserved and form noncoding RNAs known as transcribed ultraconserved regions (T-UCRs). The functional significance of T-UCRs is not apparent, but their altered expression is associated with many diseases, and thus thought to be critical for life. We presently investigated the poststroke temporal changes in the expression of T-UCRs with potential functional significance.

Methods

Male, spontaneously hypertensive rats were subjected to transient middle cerebral artery occlusion. Expression profile of T-UCRs was determined at 3, 6, and 12 hours of reperfusion using microarrays and real-time polymerase chain reaction

SUBMITTER: Mehta SL 

PROVIDER: S-EPMC5875717 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature