Specific protein carbonylation in human breast cancer tissue compared to adjacent healthy epithelial tissue.

ABSTRACT: Protein carbonylation is an irreversible post-translational modification induced by severe oxidative stress. Reactive oxygen species (ROS) are constantly produced in cells and play important roles in both cancer progression and cancer suppression. ROS levels can be higher in tumor compared to surrounding healthy tissue but ROS-induced specific protein carbonylation and its unique role in cancer progression or suppression is poorly understood. In this study, we utilized previously validated ELISA and western blot methods to analyze the total and specific protein carbonylation in flash-frozen human breast cancer and matched adjacent healthy tissue to compare relative total, and specific protein carbonylation. Mass spectrometry, two-color western, and immunoprecipitation methods were used to

SUBMITTER: Aryal B 

PROVIDER: S-EPMC5875748 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature