Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.

ABSTRACT: We have redesigned probes for in situ proximity ligation assay (PLA), resulting in more efficient localized detection of target proteins. In situ PLA depends on recognition of target proteins by pairs of antibody-oligonucleotide conjugates (PLA probes), which jointly give rise to DNA circles that template localized rolling circle amplification reactions. The requirement for dual recognition of the target proteins improves selectivity by ignoring any cross-reactivity not shared by the antibodies, and it allows detection of protein-protein interactions and post-translational modifications. We herein describe an improved design of the PLA probes -UnFold probes - where all elements required for formation of circular DNA strands are incorporated in the probes. Premature interactions between the

SUBMITTER: Klaesson A 

PROVIDER: S-EPMC5876389 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature