Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing.

ABSTRACT:

Background

The multifaceted control of gene expression requires tight coordination of regulatory mechanisms at transcriptional and post-transcriptional level. Here, we studied the interdependence of transcription initiation, splicing and polyadenylation events on single mRNA molecules by full-length mRNA sequencing.

Results

In MCF-7 breast cancer cells, we find 2700 genes with interdependent alternative transcription initiation, splicing and polyadenylation events, both in proximal and distant parts of mRNA molecules, including examples of coupling between transcription start sites and polyadenylation sites. The analysis of three human primary tissues (brain, heart and liver) reveals similar patterns of interdependency between transcription initiation and mRNA processing eve

SUBMITTER: Anvar SY 

PROVIDER: S-EPMC5877393 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature