Reduced mannosidase MAN1A1 expression leads to aberrant N-glycosylation and impaired survival in breast cancer.

ABSTRACT:

Background

Alterations in protein glycosylation have been related to malignant transformation and tumour progression. We recently showed that low mRNA levels of Golgi alpha-mannosidase MAN1A1 correlate with poor prognosis in breast cancer patients.

Methods

We analysed the role of MAN1A1 on a protein level using western blot analysis (n=105) and studied the impact of MAN1A1-related glycosylation on the prognostic relevance of adhesion molecules involved in breast cancer using microarray data (n=194). Functional consequences of mannosidase inhibition using the inhibitor kifunensine or MAN1A1 silencing were investigated in breast cancer cells in vitro.

Results

Patients with low/moderate MAN1A1 expression in tumours showed significantly shorter disease-free intervals than

SUBMITTER: Legler K 

PROVIDER: S-EPMC5877434 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature