Massive crossover elevation via combination of <i>HEI10</i> and <i>recq4a recq4b</i> during <i>Arabidopsis</i> meiosis.

ABSTRACT: During meiosis, homologous chromosomes undergo reciprocal crossovers, which generate genetic diversity and underpin classical crop improvement. Meiotic recombination initiates from DNA double-strand breaks (DSBs), which are processed into single-stranded DNA that can invade a homologous chromosome. The resulting joint molecules can ultimately be resolved as crossovers. In Arabidopsis, competing pathways balance the repair of ∼100-200 meiotic DSBs into ∼10 crossovers per meiosis, with the excess DSBs repaired as noncrossovers. To bias DSB repair toward crossovers, we simultaneously increased dosage of the procrossover E3 ligase gene HEI10 and introduced mutations in the anticrossovers helicase genes RECQ4A and RECQ4B As HEI10 and recq4a recq4b incre

SUBMITTER: Serra H 

PROVIDER: S-EPMC5877939 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature