Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities.

ABSTRACT: The gram-negative pathogen Providencia stuartii forms floating communities within which adjacent cells are in apparent contact, before depositing as canonical surface-attached biofilms. Because porins are the most abundant proteins in the outer membrane of gram-negative bacteria, we hypothesized that they could be involved in cell-to-cell contact and undertook a structure-function relationship study on the two porins of P. stuartii, Omp-Pst1 and Omp-Pst2. Our crystal structures reveal that these porins can self-associate through their extracellular loops, forming dimers of trimers (DOTs) that could enable cell-to-cell contact within floating communities. Support for this hypothesis was obtained by studying the porin-dependent aggregation of liposomes and model cells. The obse

SUBMITTER: El-Khatib M 

PROVIDER: S-EPMC5878009 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature