Human Orthobunyavirus Infections, Tefe, Amazonas, Brazil.

ABSTRACT: Several orthobunyaviruses are important arthropod-borne pathogens, responsible for a variety of diseases in humans, from acute febrile illness to encephalitis. We collected serum samples from a series of dengue suspected cases in Tefé, a mid-size city located in the interior of the Amazonas state, Brazil. Viral RNA extraction was performed, and specimens were tested for dengue virus using RT-PCR. Thirty dengue negative samples were further tested for Mayaro virus (MAYV) and Oropouche virus (OROV) using an RT-qPCR protocol previously described. Positive samples were characterized by MegaBLAST analysis over the entire nucleotide collection of the main public databases, and also by maximum likelihood phylogenetic reconstruction of the S genome segment. We detected nine OROV or OROV-like posit

SUBMITTER: Naveca FG 

PROVIDER: S-EPMC5878103 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature