SGK1 inhibition-induced autophagy impairs prostate cancer metastasis by reversing EMT.

ABSTRACT:

Background

Despite SGK1 has been identified and characterized as a tumor-promoting gene, the functions and underlying mechanisms of SGK1 involved in metastasis regulation have not yet been investigated in cancer.

Methods

We investigated the cellular responses to GSK650394 treatment and SGK1 silencing (or overexpression) in human prostate cancer (PCa) cell lines and PC3 xenografts by wound healing assay, migration and invasion assay, western blotting, immunofluorescence and immunohistochemistry.

Results

In the present study, we found that SGK1 expression positively correlates with human prostate cancer (PCa) progression and metastasis. We show that SGK1 inhibition significantly attenuates EMT and metastasis both in vitro and in vivo, whereas overexpression of SGK1 dram

SUBMITTER: Liu W 

PROVIDER: S-EPMC5879613 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature