Cryo-EM reconstruction of AlfA from <i>Bacillus subtilis</i> reveals the structure of a simplified actin-like filament at 3.4-A resolution.

ABSTRACT: Low copy-number plasmid pLS32 of Bacillus subtilis subsp. natto contains a partitioning system that ensures segregation of plasmid copies during cell division. The partitioning locus comprises actin-like protein AlfA, adaptor protein AlfB, and the centromeric sequence parN Similar to the ParMRC partitioning system from Escherichia coli plasmid R1, AlfA filaments form actin-like double helical filaments that arrange into an antiparallel bipolar spindle, which attaches its growing ends to sister plasmids through interactions with AlfB and parN Because, compared with ParM and other actin-like proteins, AlfA is highly diverged in sequence, we determined the atomic structure of nonbundling AlfA filaments to 3.4-Å resolution by cryo-EM. The structure reveals ho

SUBMITTER: Szewczak-Harris A 

PROVIDER: S-EPMC5879667 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature