Requirement for intron structures in activating the Cd8a locus.

ABSTRACT: During differentiation of CD4⁺CD8⁺ double-positive (DP) thymocytes into the CD4^-CD8⁺ single-positive (CD8SP) thymocytes committed to the cytotoxic T cell lineage, Cd8a transcription is temporally terminated after positive selection and is subsequently reinitiated, a process known as coreceptor reversal. Despite the identification of a transcriptional enhancer in the Cd8a gene that directs reporter transgene expression specifically in CD8SP thymocytes, the molecular mechanisms controlling reactivation of the Cd8a gene are not fully understood. Here, we show that, after positive selection, hCD2 reporter expression from the Cd8a locus, which was generated by insertion of hCD2 cDNA into the first exon of the Cd8a ge

SUBMITTER: Wada H 

PROVIDER: S-EPMC5879687 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature