Online immunocapture ICP-MS for the determination of the metalloprotein ceruloplasmin in human serum.

ABSTRACT:

Objective

The human copper-protein ceruloplasmin (Cp) is the major copper-containing protein in the human body. The accurate determination of Cp is mandatory for the reliable diagnosis of several diseases. However, the analysis of Cp has proven to be difficult. The aim of our work was a proof of concept for the determination of a metalloprotein-based on online immunocapture ICP-MS. The immuno-affinity step is responsible for the enrichment and isolation of the analyte from serum, whereas the compound-independent quantitation with ICP-MS delivers the sensitivity, precision, and large dynamic range. Off-line ELISA (enzyme-linked immunosorbent assay) was used in parallel to confirm the elution profile of the analyte with a structure-selective method. The total protein elution was obse

SUBMITTER: Bernevic B 

PROVIDER: S-EPMC5879926 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature