Engineering of E. coli inherent fatty acid biosynthesis capacity to increase octanoic acid production.

ABSTRACT:

Background

As a versatile platform chemical, construction of microbial catalysts for free octanoic acid production from biorenewable feedstocks is a promising alternative to existing petroleum-based methods. However, the bio-production strategy has been restricted by the low capacity of E. coli inherent fatty acid biosynthesis. In this study, a combination of integrated computational and experimental approach was performed to manipulate the E. coli existing metabolic network, with the objective of improving bio-octanoic acid production.

Results

First, a customized OptForce methodology was run to predict a set of four genetic interventions required for production of octanoic acid at 90% of the theoretical yield. Subsequently, all the ten candidate proteins assoc

SUBMITTER: Tan Z 

PROVIDER: S-EPMC5879999 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature