Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains.

ABSTRACT: Accurate and rapid detection of bacterial plant pathogen is the first step toward disease management and prevention of pathogen spread. Bacterial plant pathogens Clavibacter michiganensis subsp. nebraskensis (Cmn), Pantoea stewartii subsp. stewartii (Pss), and Rathayibacter tritici (Rt) cause Goss's bacterial wilt and blight of maize, Stewart's wilt of maize and spike blight of wheat and barley, respectively. The bacterial diseases are not globally distributed and not present in Korea. This study adopted comparative genomics approach and aimed to develop specific primer pairs to detect these three bacterial pathogens. Genome comparison among target pathogens and their closely related bacterial species generated 15-20 candidate primer

SUBMITTER: Baek KY 

PROVIDER: S-EPMC5880354 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature