Dopamine D2 receptor upregulates leptin and IL-6 in adipocytes.

ABSTRACT: Leptin is a pro-inflammatory cytokine secreted by the adipose tissue. Dopamine D₂ receptors (D₂Rs) have anti-inflammatory effects in the brain and kidney tissues. Mouse and human adipocytes express D₂R; D₂R protein was 10-fold greater in adipocytes from human visceral tissue than subcutaneous tissue. However, the function of D₂R in adipocytes is not well understood. 3T3-L1 cells were treated with D₂-like receptor agonist quinpirole, and immunoblot and quantitative PCR were performed. Quinpirole increased the protein and mRNA expression of leptin and IL-6, but not adiponectin and visfatin (24 h). It also increased the mRNA expression of TNF-α , MCP1, and NFkB-p50. An acute increase in the protein expression of leptin and TNF-

SUBMITTER: Wang X 

PROVIDER: S-EPMC5880505 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature