Antibody-sandwich ELISA analysis of a novel blood biomarker of CST4 in gastrointestinal cancers.

ABSTRACT:

Background

Members of the cystatin family have increasingly been proven to be involved in several tumors, including gastric cancer (GC) and colorectal cancer (CRC). Cystatin S (CST4) was found to be upregulated at the gene expression level in GC cells, making it a potential novel biomarker for the early diagnosis of gastrointestinal cancer.

Materials and methods

Quantitative real-time polymerase chain reaction and Western blotting analysis were used to explore CST4 expression in gastrointestinal cancer tissues and cell lines. We purified CST4 recombinant protein and generated anti-CST4 monoclonal antibodies to develop an antibody-sandwich enzyme-linked immunosorbent assay (ELISA) analysis system for blood CST4 detection. The performance and clinical efficacy of the detection

SUBMITTER: Dou Y 

PROVIDER: S-EPMC5880518 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature