Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

ABSTRACT: Hypoxia is frequently encountered in tumors and results in the stabilization of hypoxia inducible factors (HIFs). These factors transcriptionally activate genes that allow cells to adapt to hypoxia. In cancers, hypoxia and HIFs have been associated with increased invasion, metastasis, and resistance to chemo and radiation therapy. Here we have characterized the metabolic consequences of silencing HIF-1α and HIF-2α singly or combined in MDA-MB-231 triple negative human breast cancer xenografts, using non-invasive proton magnetic resonance spectroscopic imaging (¹H MRSI) of in vivo tumors, and high-resolution ¹H MRS of tumor extracts. Tumors from all three sublines showed a significant reduction of growth rate. We identified new metabolic targets of HIF, and demo

SUBMITTER: Bharti SK 

PROVIDER: S-EPMC5880607 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature