ImmunoPET of Malignant and Normal B Cells with ⁸⁹Zr- and ¹²⁴I-Labeled Obinutuzumab Antibody Fragments Reveals Differential CD20 Internalization In Vivo.

ABSTRACT: Purpose: The B-cell antigen CD20 provides a target for antibody-based positron emission tomography (immunoPET). We engineered antibody fragments targeting human CD20 and studied their potential as immunoPET tracers in transgenic mice (huCD20TM) and in a murine lymphoma model expressing human CD20.Experimental Design: Anti-CD20 cys-diabody (cDb) and cys-minibody (cMb) based on rituximab and obinutuzumab (GA101) were radioiodinated and used for immunoPET imaging of a murine lymphoma model. Pairwise comparison of obinutuzumab-based antibody fragments labeled with residualizing (⁸⁹Zr) versus non-residualizing (¹²⁴I) radionuclides by region of interest analysis of serial PET images was conducted both in the murine lymphoma model and in huCD20TM to assess anti

SUBMITTER: Zettlitz KA 

PROVIDER: S-EPMC5880625 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature