Conditional ablation of the prorenin receptor in nephron progenitor cells results in developmental programming of hypertension.

ABSTRACT: Nephron induction during kidney development is driven by reciprocal interactions between progenitor cells (NPCs) of the cap mesenchyme (CM) and the ureteric bud (UB). The prorenin receptor (PRR) is a receptor for renin and prorenin, and an accessory subunit of the vacuolar proton pump V-ATPase. Previously, we demonstrated that conditional ablation of the PRR in Six2⁺ NPCs in mice (Six2^PRR-/- ) causes early neonatal death. Here, we identified genes that are regulated by PRR in Six2⁺ NPCs FACS-isolated from Six2^PRR-/- and control kidneys on embryonic day E15.5 using whole-genome expression analysis. Seven genes with expression in CM cells previously shown to direct kidney development, including Notch1, β-catenin, Lef1, Lhx1, J

SUBMITTER: Song R 

PROVIDER: S-EPMC5880790 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature